In recent years, the use of biomass for bioethanol production has been the object of much research. Depending on the type of biomass and processing method used, furfural or 5-hydroxymethyl furfural (compounds which are known fermentation inhibitors) are produced, In this application the SUGAR SH1821 column was used to simultaneously analyze cello-oligosaccharides along with their constitutive sugars and glucose. SH1821combines ion exclusion, ligand exchange and size exclusion modes and is recommended for the simultaneous analysis of sugars and organic acids.


Sample : 0.1 % each, 10 μL
1. Cellopentaose
2. Cellotetraose
3. Cellotriose
4. Cellobiose
5. Glucose
6. Glyceric acid
7. Acetic acid
8. 5-HMF
9. Furfural

Column       : Shodex SUGAR SH1821 (8.0 mm I.D. x 300 mm)
Eluent       : 2 mM H2SO4 aq.
Flow rate    : 0.6 mL/min
Detector     : RI
Column temp. : 60 °C