With SUGAR SH1011
and SH1821, which operate under ion exclusion
and GFC modes, the use of perchloric acid aqueous solution allows analysis of
saccharides and organic acids in a single run. By changing the concentration (pH)
of the perchloric acid aqueous solution and the column temperature, the elution
time of organic acids can be adjusted. However, when using columns such as SH1011
and SH1821 whose counter ions are of H type for the analysis of sucrose in a sample,
measurement should be performed at a temperature as low as around 15°C for
preventing the decomposition of sucrose.
Sample :
1. Sucrose
2. Citric acid
3. Glucose
4. Fructose
5. Lactic acid
6. Succinic acid
7. Formic acid
8. Acetic acid
Column : Shodex SUGAR SH1011 (8.0mmI.D. x 300mm) Eluent : 3mM HClO4 aq. Flow rate : 1.0mL/min Detector : Shodex RI Column temp. : 15°C
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