Elution Volume of Saccharides and Organic Acids (SH1011)

Elution volume of saccharides and organic acids using SUGAR SH1011 is summeraized. When the concentration of sulfuric acid in the eluent is changed, the elution volume of organic acids changes in some degree though that of saccharides changes very little. Optimal concentration of sulfuric acid should be selected according to the sample to be analyzed.

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Saccharides
Sugar alcohols		 Retention Time (min) Ratio of retention time
(b/a %)
Eluent (a) Eluent (b)
Stachyose   8.59   8.66 100.85
Isomaltotriose   8.85   8.90 100.59
Panose   8.94   8.98 100.48
Maltotriose   9.03   9.09 100.65
Gentiobiose   9.47   9.51 100.42
Isomaltose     9.63   9.69 100.64
Melibiose     9.77   9.82 100.51
Kojibiose   9.79   9.83 100.44
Maltose   9.80   9.86 100.60
Trehalose   9.82   9.87 100.53
Nigerose   9.87   9.87   99.99
Palatinose   9.96   9.75   97.91
Palatinit   9.96 10.04 100.77
Trehalulose   9.97 10.02 100.51
Lactose   9.99 10.05 100.55
Xylobiose 10.09 10.15 100.52
Maltitol 10.19 10.27 100.78
Lactitol 10.45 10.54 100.84
Glucose 11.32 11.37 100.47
Sorbose 11.54 11.58 100.32
myo-Inositol 11.69 11.75 100.47
D-Mannose 11.85 11.90 100.44
D(+)-Galactose 11.89 11.94 100.44
D(+)-Xylose 11.92 11.97 100.43
D(-)-Fructose 12.01 12.05 100.37
Mannitol 12.26 12.34 100.63
D(-)-Sorbitol 12.42 12.47 100.39
D(+)-Rhamnose 12.46 12.51 100.41
D(+)-Arabinose 12.74 12.79 100.40
D(-)-Ribose 13.01 13.04 100.22
D-Arabitol 13.07 13.14 100.57
Xylitol 13.25 13.33 100.61
D(+)-Fucose 13.35 13.40 100.40
meso-Erythritol 13.81 13.89 100.60
N-Acetyl-α-D-glucosamine 14.02 14.05 100.21
N-Acetyl-α-D-galactosamine 15.47 15.49 100.12
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Organic Acids Retention Time (min) Ratio of retention time
(b/a %)
Eluent (a) Eluent (b)
Oxalic acid   8.46   9.38 110.90
D-Glucuronic acid 10.24 10.39 101.46
Citric acid 10.25 10.48 102.26
α-Ketoglutaric acid 10.27 11.47 111.66
Maleic acid 10.28 11.77 114.46
N-Acetylneuraminic acid 10.53 10.94 103.89
Tartaric acid 10.67 11.06 103.65
Pyruvic acid 11.48 12.79 111.36
Malic acid 11.74 11.93 101.63
Malonic acid 11.95 12.53 104.84
trans-Aconitic acid 12.01 12.51 104.10
Succinic acid 13.78 13.83 100.39
DL-Lactic acid 14.73 14.86 100.85
Formic acid 15.70 15.82 100.78
Acetic acid 16.90 16.93 100.18
Adipic acid 17.32 17.42 100.59
Mesaconic acid 18.28 19.02 104.07
L-Pyroglutamic acid 19.00 19.53 102.77
Propionic acid 19.45 19.54 100.40
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Alcohol Retention Time (min) Ratio of retention time
(b/a %)
Eluent (a) Eluent (b)
Ethanol 23.43 23.52 100.38
Column
：Shodex SUGAR SH1011 (8.0 mm I.D. x 300 mm)
Eluent
：(a); 5 mM H2SO4 aq.
(b); 20 mM H2SO4 aq.
Flow rate
：0.6 mL/min
Detector
：RI
Column temp.
：60 ℃

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