Melamine (1,3,5-Triazine-2,4,6-triamine), a compound fraudulently used to falsely increase protein content in milk, is a toxic substance known to cause bladder and kidney stones in humans when chronically ingested. The discovery of melamine contamination in baby formula and in other food products has prompted the need for the development of suitable analytical methods for this substance. This chromatogram shows an application based on HILIC mode using Asahipak NH2P-50 4E.

Sample: 5 μL

  1. 1.Melamine 0.5 mg/L
Column
Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent
H2O/CH3CN=25/75
Flow rate
:1.0 mL/min
Detector
UV (230 nm)
Column temp.
30 ℃

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