Perfluorooctanesulfonic acid (PFOS) and Perfluorooctanoic acid (PFOA) rarely decompose in natural environment and thus their persistence in the environment and accumulations in living organisms are problems to be considered. They are subjects of the Stockholm Convention on Persistent Organic Pollutants.
In this application, RSpak JJ-50 2D, a multimode (reversed phase + anion exchange) column was used to analyze PFOS and PFOA. Eluents with different composition ratios of acetonitrile and ammonium hydrogen carbonate aqueous solution were tested under isocratic conditions. Plotted retention times (tR) with respect to acetonitrile ratio showed a U-shape trend. At 90 % and 30 % acetonitrile conditions, PFOS and PFOA were not detected in the used setting, respectively. Most likely, they were retained longer than 20 minutes. The higher signal to noise ratio (S/N) was achieved with the higher acetonitrile eluent. This is because presence of larger acetonitrile content promoted the desolvation at the ion source.
Sample: 1 µL, 100 ng/mL each
- PFOS;Perfluorooctanesulfonic acid
- PFOA;Perfluorooctanoic acid
- Column
- :Shodex RSpak JJ-50 2D (2.0 mm I.D. × 150 mm)
- Eluent
- :50 mM NH4HCO3 aq./CH3CN=70/30, 60/40, 50/50, 40/60, 30/70, 20/80, 10/90
- Flow rate
- :0.2 mL/min
- Detector
- :ESI-MS (SIM Negative)
- Column temp.
- :40 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Analysis of Haloacetic Acids (JJ-50 2D)
- LC/MS Analysis of Haloacetic Acids (JJ-50 2D)
- Melamine (1) (NH2P-50 4E)
- Melamine (2) (NH2P-50 2D)
- LC/MS Analysis of Melamine and Cyanuric Acid (NH2P-40 2D)
- Melamine and Related Substances (VG-50 4D)
- Analysis of PFAS (VT-50 2D)
- Analysis of PFAS According to Japanese Industrial Standards Method (JJ-50 2D)
- LC/MS Analysis of Organic Arsenic Compound (SI-35 2B)