Effects of Eluent Composition Ratios on PFOS and PFOA Analysis (JJ-50 2D)

Perfluorooctanesulfonic acid (PFOS) and Perfluorooctanoic acid (PFOA) rarely decompose in natural environment and thus their persistence in the environment and accumulations in living organisms are problems to be considered. They are subjects of the Stockholm Convention on Persistent Organic Pollutants.
In this application, RSpak JJ-50 2D, a multimode (reversed phase + anion exchange) column was used to analyze PFOS and PFOA. Eluents with different composition ratios of acetonitrile and ammonium hydrogen carbonate aqueous solution were tested under isocratic conditions. Plotted retention times (tR) with respect to acetonitrile ratio showed a U-shape trend. At 90 % and 30 % acetonitrile conditions, PFOS and PFOA were not detected in the used setting, respectively. Most likely, they were retained longer than 20 minutes. The higher signal to noise ratio (S/N) was achieved with the higher acetonitrile eluent. This is because presence of larger acetonitrile content promoted the desolvation at the ion source.

Sample: 1 µL, 100 ng/mL each

  1. PFOS;Perfluorooctanesulfonic acid
  2. PFOA;Perfluorooctanoic acid
Column
Shodex RSpak JJ-50 2D (2.0 mm I.D. × 150 mm)
Eluent
50 mM NH4HCO3 aq./CH3CN=70/30, 60/40, 50/50, 40/60, 30/70, 20/80, 10/90
Flow rate
0.2 mL/min
Detector
ESI-MS (SIM Negative)
Column temp.
40 ℃

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