Cyanuric acid is a structural analogue of melamine, and thus sometimes detected as an impurity of melamine. In this application, polymer-based amino column, Asahipak NH2P-40 2D, was used to separate melamine and cyanuric acid using HILIC mode. Hydrophilic compounds are retained at a high concentration acetonitrile under HILIC mode condition. This is advantageous for the high sensitivity analysis by ESI-MS.
*Sales of Asahipak NH2P-40 2D used in this application is discontinued. Please consider trying Asahipak NH2P-50 2D.

Sample: 5 μL
500 ng/mL each

1. 1.Melamine
2. 2.Cyanuric acid