Cyanuric acid is a structural analogue of melamine, and thus sometimes detected as an impurity of melamine. In this application, polymer-based amino column, Asahipak NH2P-40 2D, was used to separate melamine and cyanuric acid using HILIC mode. Hydrophilic compounds are retained at a high concentration acetonitrile under HILIC mode condition. This is advantageous for the high sensitivity analysis by ESI-MS.
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Sample : 500 ng/mL each, 5 μL
1. Melamine
2. Cyanuric acid

Column       : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
Eluent       : 50 mM Ammonium formate aq./CH3CN=25/75
Flow rate    : 0.2 mL/min
Detector     : Melamine; ESI-MS/MS
               Cyanuric acid; ESI-MS
Column temp. : 30 °C