Solvent Exchange Procedures for Shodex GPC Series Columns

To exchange/replace the solvent currently filled in the Shodex GPC series columns, follow the below solvent exchange procedures.

NoteWhen replacing the solvent, set the column temperature at 40 to 60 ℃ and start the system at less than half of the recommended flow rate.
Recommended solvent volume to introduce at each step is 3 to 5 times of the column volume.

Before solvent replacement

Solvent replacement

  1. (1)When replacing the current solvent with a miscible solvent, first, introduce 1:1 mixture of the current solvent and the new solvent, and then replace it with 100 % of the new solvent.
    e.g. When replacing THF with Chloroform
    　　First introduce 1:1 mixture of THF and Chloroform, and then introduce 100 % Chloroform.
  2. (2)When replacing the current solvent with a solvent with low miscibility/solubility to the current solvent, first use a solvent that is miscible/soluble to both eluents, and then replace it with the new solvent.
    e.g. When replacing THF with “HFIP with CF3COONa”
    　　First follow the example in procedure (1), and then replace 100 % HFIP with “HFIP with CF3COONa”.

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