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N-Acetylchitooligosaccharides (2) (VN-50 4D)

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N-Acetylchitooligosaccharides (Chitin oligosaccharides) were separated using HILICpak VN-50 4D, a polymer-based HILIC column, with evaporative light scattering detector (ELSD). Oligosaccharides up to a heptamer could be analyzed in this sample.


Sample : 10μL
N-Acetylchitooligosaccharides 5mg/mL (in 50% CH3CN)
1. N-Acetyl-D-glucosamine
2. di-N-Acetylchitobiose
3. tri-N-Acetylchitotriose
4. tetra-N-Acetylchitotetraose
5. penta-N-Acetylchitopentaose
6. hexa-N-Acetylchitohexaose
7. hepta-N-Acetylchitohexaose


Column       : Shodex HILICpak VN-50 4D (4.6mmI.D. x 150mm)
Eluent       : (A); H2O (B); CH3CN
               Linear gradient ; (B%) 80% to 65% (0 to 10min)
Flow rate    : 1.0mL/min
Detector     : ELS
Column temp. : 60°C

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