Oligosaccharides of hydrolyzed dextran were separated using HILICpak VN-50 4D by isocratic elution. VN-50 4D provides much better separation for oligosaccharides than conventional HILIC columns and each oligosaccharide is well defined between each chain. Furthermore, longer oligosaccharides can be detected with VN-50 4D.
*To improve the separation of mono- and di- saccharides, the ratio of acetonitrile can be increased.
Sample : 10 µL
Hydrolyzed dextran 0.5 %
(in H2O/CH3CN=50/50)
Column : Shodex HILICpak VN-50 4D
Shodex Asahipak NH2P-50 4D (4.6 mm I.D. x 150 mm each)
Eluent : (VN-50 4D); H2O/CH3CN=40/60
(NH2P-50 4D); H2O/CH3CN=50/50
Flow rate : 0.5 mL/min
Detector : RI (small cell volume)
Column temp. : 40 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Hydrolyzed Dextran (2) (KS-802)
- Hydrolyzed Dextran (3) (NH2P-50 4E)
- Hydrolyzed Dextran (5) (KS-802)
- Hydrolyzed Dextran (7) (VN-50 4D by Gradient Elution)
- Isomaltooligosaccharides (NH2P-50 4E)
- Isomaltooligosaccharides (2) (VG-50 4E)
- Maltooligosaccharides (NH2P-50)
- Maltooligosaccharides (2) (VG-50 4E)
- Comparison of maltooligosaccharides separation
- N-Acetylchitooligosaccharides (1) (NH2P-50 4E)
- N-Acetylchitooligosaccharides (2) (VN-50 4D)
- Chitosan-Oligosaccharides (Chitooligosaccharides) (1) (NH2P-50 4E)
- Chitosan-Oligosaccharides (Chitooligosaccharides) (2) (Asahipak NH2P-50 4E)
- Chitosan-Oligosaccharides (Chitooligosaccharides) (3) (VN-50 4D)
- Cyclodextrins (Comparison of GS-220 HQ and KS-802)
- Analysis of Cyclodextrins (NH2P-50 4E)
- Analysis of Cyclodextrins According to Japan's Specifications and Standards for Food Additives Method (KS-802)
- Short-Chain Amylose (1) (NH2P-50 10E)
- Short-Chain Amylose (2) (ODP-50 6D)
- Hydrolyzed Starch (2) (KS-802)
- Sweetener (KS-802)
- Effects of Flow Rate (SC1211)
- Fructooligosaccharide Syrup (NH2P-50 4E)
- Analysis of Fructooligosaccharide Components (KS-802 + KS-801)
- Analysis of Fructooligosaccharides in Accordance to the Food System for Specified Health Use (VG-50 4E)
- Starch Syrup
- Low-Molecular-Weight Water Soluble Dietary Fiber (GS-220 HQ, KS-802)
- Xylooligosaccharides (1) (KS-801)
- Xylooligosaccharides (2) (VN-50 4D)
- Xylooligosaccharides (3) (SH1011)
- Degradation Product of Cellulose with Cellulase (PcCel45A) Digestion
- Oligosaccharide in Soy Bean (KS-802 + KS-801)
- Analysis of Trisaccharides (1) (NH2P-40 3E)
- Analysis of Trisaccharides (2) (VG-50 4E)
- Galactooligosaccharides (1) (VG-50 4E)
- Galactooligosaccharides (2) (KS-801, NH2P-50 4E)
- Hydroxypropyl-β-cyclodextrin (SB-802.5 HQ)
- Analysis of Maltodextrin (VN-50 4D)
- Separation of Kestose and Maltose (KS-802)