The content (%) of galactooligosaccharides and the content (%) of 4'-galactosyllactose should be determined in standardized FOSHU (Food for Specified Health Uses) in Japan. According to the regulations, galactooligosaccharides should be analyzed using an ion exclusion column, but the quantitative value contains lactose. Therefore, lactose is quantified using a HILIC mode column, the value obtained by subtracting the quantitative value of lactose from the above quantitative value is taken as the quantitative value of galactooligosaccharides. In this application, galactooligosaccharides standards ware analyzed using SUGAR KS-801 as an ion exclusion column and Asahipak NH2P-50 4E as a HILIC mode column.


Sample : 10 μL
Galactooligosaccharides std. 2.5 mg/mL
1. 4'-Galactosyllactose
2. Lactose

Columns      : (Left) Shodex SUGAR KS-801 (8.0 mm I.D. x 300 mm)
               (Right) Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)　　　　　　　　　　
Eluent       : (Left) H2O
               (Right) CH3CN/H2O=70/30
Flow rate    : (Left) 0.5 mL/min
               (Right) 1.0 mL/min
Detector     : RI
Column temp. : (Left) 70 °C
               (Right) 25 °C