Chitosan-Oligosaccharides (Chitooligosaccharides) (3) (VN-50 4D)

Chitosan-oligosaccharides were separated using HILICpak VN-50 4D, a polymer-based HILIC column, with an evaporative light scattering detector (ELSD). Oligosaccharides up to an octamer could be analyzed in this sample.

Sample: 10 μL
Glucosamine hydrochloride 0.1 mg/mL (in 50 % CH3CN)
Chitosan-oligosaccharides 5 mg/mL (in 50 % CH3CN)

  1. 1.Cl-
  2. 2.D-Glucosamine
  3. 3.Chitobiose
  4. 4.Chitotriose
  5. 5.Chitotetraose
  6. 6.Chitopentaose
  7. 7.Chitohexaose
  8. 8.Chitoheptaose
  9. 9.Chitooctaose
Column
Shodex HILICpak VN-50 4D (4.6 mm I.D. x 150 mm)
Eluent
(A); 0.1 % NH3 aq. (B); CH3CN
Linear gradient; (B %) 75 % to 60 % (0 to 10 min), 60 % (10 to 15 min)
Flow rate
1.0 mL/min
Detector
ELSD
Column temp.
40 ℃

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