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Chitosan-Oligosaccharides (Chitooligosaccharides) (3) (VN-50 4D)

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Chitosan-oligosaccharides were separated using HILICpak VN-50 4D, a polymer-based HILIC column, with an evaporative light scattering detector (ELSD). Oligosaccharides up to an octamer could be analyzed in this sample.


Sample : 10µL
Glucosamine hydrochloride 0.1mg/mL (in 50% CH3CN)
Chitosan-oligosaccharides 5mg/mL (in 50% CH3CN)
1. Cl-
2. D-Glucosamine
3. Chitobiose
4. Chitotriose
5. Chitotetraose
6. Chitopentaose
7. Chitohexaose
8. Chitoheptaose
9. Chitooctaose


Column       : Shodex HILICpak VN-50 4D (4.6mmI.D. x 150mm)
Eluent       : (A); 0.1% NH3 aq. (B); CH3CN
               Linear gradient ; (B%) 75% to 60% (0 to 10min),
                                      60% (10 to 15min)
Flow rate    : 1.0mL/min
Detector     : ELS
Column temp. : 40°C 

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