GABA (γ-aminobutyric acid) is a functional ingredient in foods and has blood pressure suppressing and relaxing effects. It is one of the compounds registered as the Japanese functional ingredient in many numbers. In this application, a commercial supplement containing GABA was analyzed by a polymer-based amino column, Asahipak NH2P-50 4E, using its HILIC mode. Supplements, which do not contain many interfering matrixes can be analyzed without using a post-column method. It provided a good linear calibration curve in the concentration range 1 to 1,000 mg/L.

Sample Preparation

1. (1)Take out the content (powder) from a commercial GABA supplement hard capsule.
2. (2)Dissolve 5 mg of the removed powder in 25 mL of eluent.
3. (3)Filter using a 0.45-µm membrane filter and use it as the injection sample.

Sample: 5 µL
GABA standard solution 1000 mg/L
Commercial supplement

1. 1.GABA (γ-Aminobutyric acid)