Amino acids were analyzed using a cation chromatography column, IC YS-50. YS-50 is packed with polyvinyl alcohol with carboxyl functional group. Amino acids are separated by reversed-phase mode and ionic interaction, as RSpak NN-814 does.


Sample : 5 μL
1. Aspartic acid 0.8 mg/mL
2. Glutamic acid 0.8 mg/mL
3. Glycine 0.8 mg/mL
4. Valine 1.0 mg/mL
5. Methionine 1.0 mg/mL
6. Isoleucine 1.0 mg/mL
7. GABA 1.0 mg/mL
8. Phenylalanine 1.0 mg/mL
9. Ornithine 1.0 mg/mL
10. Histidine 1.0 mg/mL
11. 1-Methylhistidine 1.0 mg/mL
12. Arginine 1.0 mg/mL

Column       : Shodex IC YS-50 (4.6 mm I.D. x 125 mm)
Eluent       : 6 mM H3PO4 aq.
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 40 °C