Theanine is a type of amino acid which is contained in tea, and is a component related to umami and sweetness. In addition, theanine has reported relaxation effects such as improving sleep quality. Here, theanine as well as various amino acids contained in tea were separated using Asahipak NH2P-50 4E, a polymer-based amino column, under HILIC mode conditions.


Sample : 20 μL
1. Theanine 10 μg/mL
2. Threonine 100 μg/mL
3. Glutamic acid 50 μg/mL
4. Serine 100 μg/mL
5. Aspartic acid 50 μg/mL
6. Arginine 50 μg/mL
(in eluent)

Column       : Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
Eluent       : 20 mM NaH2PO4 + 30 mM H3PO4 aq./CH3CN=30/70
Flow rate    : 1.0 mL/min
Detector     : UV (210 nm)
Column temp. : 30 °C