Amino acids were analyzed using IC YS-50 for cations. YS-50 is packed with polyvinyl alcohol with carboxyl functional group. Amino acids are separated by reversed-phase mode and ionic interaction, as NN-814 column does Alanine and Glycine elute at the same elution time under this condition.
See also Amino Acids (2) (NN-814) .


Sample : 0.02 % each, 5 μL
1. Aspartic acid
2. Glycine
3. Valine
4. Methionine
5. Isoleucine

Column       : Shodex IC YS-50 (4.6 mm I.D. x 125 mm)
Eluent       : 2 mM Acetic acid aq.
Flow rate    : 1.0 mL/min
Detector     : RI
Column temp. : 40 °C