グアニンとトリプトファンの分離を3種類のカラムRSpak DM-614、NN-814、DE-613を用いて行いました。
Sample : 1. Guanine, 2. Tryptophan
Columns : (left); Shodex RSpak DM-G 4A (4.6 mm I.D. x 10 mm) + DM-614 (6.0 mm I.D. x 150 mm) (Center); Shodex RSpak NN-G (6.0 mm I.D. x 50 mm) + NN-814 (8.0 mm I.D. x 250 mm) (Right); Shodex RSpak DE-G 4A (4.6 mm I.D. x 10 mm) + DE-613 (6.0 mm I.D. x 150 mm) Eluent : (Left); 0.3 M K2HPO4(pH8.6) (Center); 0.1 M NaH2PO4(pH3.0 adjusted by H3PO4) (Right); 0.23 M K2HPO4 aq./CH3OH=80/20 Flow rate : (Left); 0.8 mL/min, (Center); 1.0 mL/min, (Right); 0.8 mL/min Detector : (Left); UV(278 nm), (Center); RI, (Right); UV(278 nm) Column temp.: (Left); 30 ℃, (Center); 50 ℃, (Right); 30 ℃