Anthocyanidins are polyphenols found in grape skins and blueberries. They have antioxidant and anti-inflammatory effects, which are expected to improve vision and nerve health. In this application, we performed separation of six major anthocyanidins using Silica C18 M 4D, a silica-based reversed-phase chromatography column. Six anthocyanidins were analyzed within 20 minutes using an isocratic elution.
Sample: 10 μL
- 1.Delphinidin 20 μg/mL
- 2.Cyanidin 10 μg/mL
- 3.Petunidin 40 μg/mL
- 4.Pelargonidin 10 μg/mL
- 5.Peonidin 20 μg/mL
- 6.Malvidin 20 μg/mL
- Column
- :Shodex Silica C18M 4D (4.6 mm I.D. x 150 mm)
- Eluent
- :5 % HCOOH/CH3CN/CH3OH=80/10/10
- Flow rate
- :1.0 mL/min
- Detector
- :VIS (520 nm)
- Column temp.
- :40 ℃
Sample Name Index
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