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Analysis of Ellagic Acid in a Supplement (C18U 2D)

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Ellagic acid, a type of natural polyphenol contained in pomegranate and raspberries, is a functional food component. It has various effects such as reduction of body fat, blood triglyceride, and visceral fat, or works as antioxidant, antiviral, and skin whitening reagents. In this application, ellagic acid in a commercial supplement was analyzed using a UHPLC column, C18U 2D. A good linear calibration was obtained for the concentration range of 0.1 to 5 mg/L.

(Sample Preparation)
1) Grind a dietary supplement tablet in a mortar to make it into powders.
2) Precisely measure 0.2 g of the powder and add 15 mL dimethyl sulfoxide.
3) Sonicate for 10 minutes and then centrifuge at 2500 rpm for 5 minutes.
4) Collect the supernatant. Add another 15 mL dimethyl sulfoxide and repeat (3).
5) Fill up the collected supernatant with dimethyl sulfoxide to make up the volume to 50 mL.
6) Take 1 mL out from (5) and dilute with dimethyl sulfoxide to make up the volume to 10 mL. Use it as an injection sample.


Sample : 2 μL each
Standard 1 mg/L (in DMSO)
1. Ellagic acid


Column       : Shodex C18U 2D (2.0 mm I.D. x 150 mm)
Eluent       : 0.1 % H3PO4 aq./CH3OH=60/40
Flow rate    : 0.2 mL/min
Detector     : UV (254 nm)
Column temp. : 40 °C

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