Tea catechins are functional food components which have various effects such as anti-cancer and antioxidant effects, and inhibit blood pressure increase, blood sugar increase, and body fat accumulation. In this application, tea catechins in a commercial regular green tea and a commercial green tea claimed to be a functional beverage were analyzed using a UHPLC column, C18U 2D. By using the C18U 2D, eight types of tea chatechins were analyzed under isocratic mode within 10 minutes. A good linear calibration was obtained for the concentration range of 1 to 100 mg/L.

(Sample Preparation)
1) Dilute the green tea 1+4 with water.
2) Filer using a 0.45-µm membrane filter and use it as an injection sample.


Sample : 20 mg/L each, 5 μL
1. Gallocatechin
2. Epigallocatechin
3. Catechin
4. Epicatechin
5. Epigallocatechingallate
6. Gallocatechingallate
7. Epicatechingallate
8. Catechingallate

Column       : Shodex C18U 2D (2.0 mm I.D. x 150 mm)
Eluent       : 20 mM H3PO4 aq./CH3CN=80/20
Flow rate    : 0.3 mL/min
Detector     : UV (210 nm)
Column temp. : 30 °C