Analysis of Anthocyanidins (C18M 4D)

Anthocyanidins are polyphenols found in grape skins and blueberries. They have antioxidant and anti-inflammatory effects, which are expected to improve vision and nerve health. In this application, we performed separation of six major anthocyanidins using Silica C18 M 4D, a silica-based reversed-phase chromatography column. Six anthocyanidins were analyzed within 20 minutes using an isocratic elution.

Sample: 10 μL

  1. 1.Delphinidin 20 μg/mL
  2. 2.Cyanidin 10 μg/mL
  3. 3.Petunidin 40 μg/mL
  4. 4.Pelargonidin 10 μg/mL
  5. 5.Peonidin 20 μg/mL
  6. 6.Malvidin 20 μg/mL
Dissolved in 1 % TFA/CH3OH=30/70
chromatogram of Anthocyanidins
Shodex Silica C18M 4D (4.6 mm I.D. x 150 mm)
5 % HCOOH/CH3CN/CH3OH=80/10/10
Flow rate
1.0 mL/min
VIS (520 nm)
Column temp.
40 ℃

Sample Name Index

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

Product Name Index