Glyceryl tristearate is a type of emulsifier and is widely used mainly in pharmaceuticals and cosmetics. According to the United States Pharmacopeia and the National Formulary (USP 43-NF 38*), "ASSAY" for "Glyceryl Tristearate" should be carried out with a 7.5 mm (I.D.) x 60 cm (length) column filled with L21 packing material. Use of two GPC KF-802 columns, 8.0 mm (I.D.) x 30 cm (length) each, filled with L21 packing material in series confirmed the requirements were met.

System suitability requirementsResolution between monoglycerides and disterearates peaks (System suitability solution): ≥ 1.0
Relative standard deviation of monoglycerides peak (System suitability solution): ≤ 2.0%

*The version at the time of the application acquisition.

Sample: 40 µL
(Sample solution) 8 mg/mL Glyceryl Tristearate in THF
(System suitability solution) 40 mg/mL Glyceryl Distearate in THF

1. 1.Triglycerides
2. 2.Diglycerides
3. 3.Monoglycerides
4. 4.Glycerin