Carnitine and Trimethyl glycine were analyzed by Asahipak NH2P-40 2D a polymer-based amino column under HILIC mode. Hydrophilic compounds are retained at a high concentration of acetonitrile under HILIC mode conditions. This is advantageous for high sensitivity analysis by LC/MS.
*Sales of Asahipak NH2P-40 2D used in this application is discontinued. Please consider trying Asahipak NH2P-50 2D.
Sample: 5 μL
5 ng/mL each (in H2O/CH3CN=25/75)
- 1.Trimethyl glycine
- 2.Carnitine
- Column
- ：Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm)
- Eluent
- ：50 mM HCOONH4 aq./CH3CN=20/80
- Flow rate
- ：0.2 mL/min
- Detector
- ：ESI-MS (SIM Positive)
- Column temp.
- ：30 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Analysis of Preservatives in Accordance with “Analysis Method of Food Additives in Foods” in Japan (C18M 4E)
- Preservatives (2) (DE-613)
- Preservatives (4) (DE-413)
- Preservatives (DS-413)
- Food Yellow No.4 (Tartrazine) (ODP-50 4D)
- Betaine (NH2P-50 4E)
- 2-Phenoxyethanol (ODP-50 4D)
- Preservatives (ODP-50 4D)
- Polyphosphates (2) (SB-802.5 HQ)
- Polyphosphates (3) (GS-220 HQ)
- Polyphosphates (4) (GS-220 HQ)
- Analysis of Moisturizing Agents (SC1211)
- Benzoates (DE-413)
- Alkylbenzenes (1) (DS-613)
- Alkylbenzenes (2) (DS-613)
- Alkylbenzenes (4) (DE-413)
- Alkylbenzenes (DS-413)
- Xylenols (DS-413)
- Polycyclic Aromatic Hydrocarbons (DE-413)
- Phenols (2) (DE-413)
- Chlorobenzenes (DE-613)
- Iodobenzene Diacetate (K-801)
- Anilines (ODP-50 6D)
- Durability of ODP Column with Alkaline Eluent (ODP-50 4D)
- Imidazoles (ODP2 HP-4E)
- Analysis of Glyceryl Stearate According to USP-NF Method (KF-802)
- Analysis of Glyceryl Distearate According to USP-NF Method (KF-802)
- Analysis of Glyceryl Tristearate According to USP-NF Method (KF-802)
- Analysis of Glyceryl Monooleate According to USP-NF Method (KF-802)
- Analysis of Glyceryl Monolinoleate According to USP-NF Method (KF-802)