Imidazoles were analyzed using ODP2 HP-4E.


Sample : 0.1 % each, 10 μL
1. Imidazole
2. 2-Methylimidazole
3. 4-Methylimidazole


Column       : Shodex ODP2 HP-4E (4.6 mm I.D. x 250 mm)
Eluent       : 10 mM Na2HPO4 aq./CH3CN=90/10
Flow rate    : 0.8 mL/min
Detector     : UV (220 nm)
Column temp. : 40 °C

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