Please read the column cleaning method for your column.
PROTEIN KW-800 series, KW400-4F series, PROTEIN LW-803 and PROTEIN LW-403 4D
For an efficient cleaning, reverse the flow direction.
| Product name | Flow rate | Product name | Flow rate | Product name | Flow rate |
|---|---|---|---|---|---|
| PROTEIN KW-802.5 | ≤ 0.5 mL/min | PROTEIN KW-803 | ≤ 0.5 mL/min | PROTEIN KW-804 | ≤ 0.5 mL/min |
| KW402.5-4F | ≤ 0.15 mL/min | KW403-4F | ≤ 0.15 mL/min | KW404-4F | ≤ 0.15 mL/min |
| KW405-4F | ≤ 0.15 mL/min | PROTEIN LW-803 | ≤ 0.5 mL/min | PROTEIN LW-403 4D | ≤ 0.15 mL/min |
Method 1: Adsorption of ionic (basic) compounds
Introduce the eluent with a salt concentration of 0.5 M or an acidic aqueous solution of pH 3. Recommended solvent volume to introduce is 5 to 10 times of the column volume.
Method 2: Adsorption of hydrophobic compounds
Introduce the eluent with 10 to 20 % (v/v) organic solvent (acetonitrile or methanol). Recommended solvent volume to introduce is 5 to 10 times of the column volume.
- (Attention)
- Proteins adsorbed on the column may precipitate by introducing an eluent containing an organic solvent.
- Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.
OHpak SB-800 HQ series, OHpak SB-2000 series and OHpak LB-800 series
For an efficient cleaning, reverse the flow direction.
| Product name | Flow rate | Product name | Flow rate | Product name | Flow rate |
|---|---|---|---|---|---|
| OHpak SB-802 HQ | ≤ 0.5 mL/min | OHpak SB-802.5 HQ | ≤ 0.5 mL/min | OHpak SB-803 HQ | ≤ 0.5 mL/min |
| OHpak SB-804 HQ | ≤ 0.5 mL/min | OHpak SB-805 HQ | ≤ 0.5 mL/min | OHpak SB-806 HQ | ≤ 0.5 mL/min |
| OHpak SB-806M HQ | ≤ 0.5 mL/min | OHpak SB-807 HQ | ≤ 0.5 mL/min | OHpak SB-2002 | ≤ 1.5 mL/min |
| OHpak SB-2002.5 | ≤ 1.5 mL/min | OHpak SB-2003 | ≤ 1.5 mL/min | OHpak SB-2004 | ≤ 1.5 mL/min |
| OHpak SB-2005 | ≤ 1.5 mL/min | OHpak SB-2006 | ≤ 1.5 mL/min | OHpak SB-2006M | ≤ 1.5 mL/min |
| OHpak LB-802.5 | ≤ 0.5 mL/min | OHpak LB-803 | ≤ 0.5 mL/min | OHpak LB-804 | 0.5 mL/min |
| OHpak LB-805 | ≤ 0.5 mL/min | OHpak LB-806 | ≤ 0.5 mL/min | OHpak LB-806M | 0.5 mL/min |
Method 1: Adsorption of hydrophobic compounds (when using aqueous eluent)
Introduce the eluent with a higher salt concentration. Recommended solvent volume to introduce is 5 to 10 times of the column volume.
Method 2: Adsorption of ionic compounds
Introduce the eluent with a high polar organic solvent (acetonitrile or methanol). Recommended solvent volume to introduce is 5 to 10 times of the column volume.
- (Attention)
- Keep the polar organic solvent and salt concentrations within the concentrations stated in the Operation Manual "List of Applicable Solvents".
- Keeping the washing solution in the column for a long time will lead to column deterioration. Please replace the washing solution with the eluent immediately after cleaning.