High Performance Semi-micro Aqueous SEC (GFC) Columns: KW400

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KW400	Reduced packing material particle size enhances column performance Three to four-fold higher sensitivity than KW-800 series KW405-4F is applicable analyzing samples with molecular weight above 1,000,000 Fulfills USP-NF L20, L33, and L59 requirements

(High performance semi-micro columns) [Housing material: SUS]
KW400 series is recommended to be used with semi-micro type devices. Scroll

Product code	Product name	*Plate number (TPN/column)	Particle size (μm)	Column size (mm) I.D. x Length
F6989201	KW402.5-4F	≥ 35,000	3	4.6 x 300
F6989202	KW403-4F	≥ 35,000	3	4.6 x 300
F6989203	KW404-4F	≥ 25,000	5	4.6 x 300
F6989204	KW405-4F	≥ 25,000	5	4.6 x 300
F6700132	KW400G-4A	(guard column)	5	4.6 x 10

* Measured with uridine

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Product name	Maximum pressure (MPa/Column)	Flow rate (mL/min)		Temperature range (℃)	pH range	Shipping solvent
Product name	Maximum pressure (MPa/Column)	Recommended	Maximum	Temperature range (℃)	pH range	Shipping solvent
KW402.5-4F	10	0.35	0.5	5 - 45	3 - 7.5	H₂O
KW403-4F	10	0.35	0.5	5 - 45	3 - 7.5	H₂O
KW404-4F	10	0.35	0.5	5 - 45	3 - 7.5	H₂O
KW405-4F	10	0.35	0.5	5 - 45	3 - 7.5	H₂O
KW400G-4A	-	-	-	5 - 45	3 - 7.5	H₂O

Target molecular weight range and exclusion limitPlease use the following tables for reference purposes only when selecting columns. Scroll

Product name	Measured with protein (eluent: phosphate buffer)		Measured with pullulan (eluent: ultrapure water)
Product name	Target molecular weight range	Exclusion limit	Target molecular weight range	Exclusion limit
KW402.5-4F	5,000 - 70,000	150,000	2,000 - 50,000	60,000
KW403-4F	10,000 - 500,000	600,000	5,000 - 100,000	80,000
KW404-4F	30,000 - *(4,000,000)	*(4,000,000)	20,000 - 300,000	400,000
KW405-4F	200,000 - *(20,000,000)	*(20,000,000)	100,000 - 700,000	1,300,000

*( ) Estimated value

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.