High Performance Semi-micro Aqueous SEC (GFC) Columns: KW400

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KW400
  • Reduced packing material particle size enhances column performance
  • Three to four-fold higher sensitivity than KW-800 series
  • KW405-4F is applicable analyzing samples with molecular weight above 1,000,000
  • Fulfills USP-NF L20, L33, and L59 requirements
(High performance semi-micro columns)   [Housing material: SUS]
* KW400 series is recommended to be used with semi-micro type devices. Scroll
Product code Product name *Plate number
(TPN/column)
Particle size (μm) Column size (mm)
I.D. x Length
F6989201 KW402.5-4F ≥ 35,000 3 4.6 x 300
F6989202 KW403-4F ≥ 35,000 3 4.6 x 300
F6989203 KW404-4F ≥ 25,000 5 4.6 x 300
F6989204 KW405-4F ≥ 25,000 5 4.6 x 300
F6700132 KW400G-4A (guard column) 5 4.6 x   10
* Measured with uridine
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Product name Maximum pressure
(MPa/Column)
Flow rate (mL/min) Temperature range
(℃)
pH range Shipping solvent
Recommended Maximum
KW402.5-4F
- KW405-4F
10 0.35 0.5 5 - 45 3 - 7.5 H2O
KW400G-4A - - - 5 - 45 3 - 7.5 H2O
Target molecular weight range and exclusion limitPlease use the following tables for reference purposes only when selecting columns. Scroll
Product name Measured with protein (eluent: phosphate buffer) Measured with pullulan (eluent: ultrapure water)
Target molecular weight range Exclusion limit Target molecular weight range Exclusion limit
KW402.5-4F 5,000 - 70,000      150,000 2,000 - 50,000       60,000
KW403-4F 10,000 - 500,000      600,000 5,000 - 100,000      80,000
KW404-4F 30,000 - *(4,000,000) *(4,000,000) 20,000 - 300,000    400,000
KW405-4F 200,000 - *(20,000,000) *(20,000,000) 100,000 - 700,000 1,300,000
*( ) Estimated value

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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General Information on Packed Columns

introduction of column cleaning procedure and column test conditions etc.