Separation of Nitrite and Nitrate Ions (NH2P-50 4E)

Inorganic ions are generally monitored using an electrical conductivity detector. However, inorganic ions that have UV absorption, such as nitrite and nitrate ions, can also be detected using a UV detector. Thus, they can be analyzed without using an ion chromatography system nor an ion chromatography column. In this application, nitrite and nitrate ions were separated using a polymer-based amino column Asahipak NH2P-50 4E.

Sample: 10 µL, 10 mg/L each

  1. 1.NO2-
  2. 2.NO3-
Chromatogram of nitrite and nitrate
Shodex Asahipak NH2P-50 4E (4.6 mm I.D. x 250 mm)
1.79 g Na2HPO4 ・ 12 H2O + 0.78 g NaH2PO4 ・ 2 H2O + 14.04 g NaClO4 ・ H2O in 1 L H2O
Flow rate
:0.8  mL/min
UV (210 nm)
Column temp.
40 ℃

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Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

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