Nitrite and ammonium thiocyanate (thiocyanate compounds) are known cause of plant growth disorders. According to Japanese Testing Methods for Fertilizers (2016*), "High-performance liquid chromatography method" under "5.8 Ammonium thiocyanate (thiocyanate compound)" and "5.9 Nitrite" should be carried out with a column filled with polyvinyl alcohol or silica gels modified with amino groups. The method mentions that this analysis is applicable to fertilizers, and Asahipak NH2P-50 4E as a suitable column. In this application, nitrite and ammonium thiocyanate were analyzed using NH2P-50 4E. The calibration curves for nitrite and thiocyanate showed high linearity over the concentration range of 1 to 20 µg/mL. 

*The version at the time of the application acquisition.

Sample: 10 µL
10 µg/mL each

1. 1.Nitrite
2. 2.Thiocyanate

 

1.