Physical chemistry properties of solvents which are commonly used for SEC analysis is shown below.
Solvents | Chemical formula | Boiling point (℃) |
Viscosity (cP, 25 ℃) |
Transmission limit (nm) |
Refractive index (nD25) |
---|---|---|---|---|---|
Acetic acid | CH3COOH | 118 | 1.1 | - | 1.372 |
Acetonitrile | CH3CN | 82 | 0.34 | 190 | 1.344 |
Acetone | CH3COCH3 | 56 | 0.30 | 330 | 1.359 |
Carbon tetrachloride | CCl4 | 77 | 0.90 | 265 | 1.466 |
Chloroform | CHCl3 | 61 | 0.53 | 245 | 1.443 |
Cyclohexane | C6H12 | 81 | 0.90 | 200 | 1.427 |
Dichloroethane | CH2ClCH2Cl | 83 | 0.78 | 228 | 1.445 |
Dichloromethane | CH2Cl2 | 40 | 0.41 | 233 | 1.424 |
Dimethylformamide | (CH3)2NOCH | 153 | 0.80 | 268 | 1.428 |
Dimethylsulfoxide | (CH3)2SO | 189 | 2.00 | 268 | 1.477 |
Dioxane | C4H8O2 | 101 | 1.2 | 215 | 1.422 |
Ethanol | C2H5OH | 78 | 1.08 | 210 | 1.361 |
Ethyl acetate | CH3COOC2H5 | 77 | 0.43 | 256 | 1.370 |
Ethylene glycol | HOCH2CH2OH | 182 | 16.5 | - | 1.431 |
n-Hexane | CH3(CH2)4CH3 | 69 | 0.30 | 190 | - |
Isopropyl alcohol | CH3CH(OH)CH3 | 82 | 1.9 | 205 | 1.384 |
Isopropyl ether | (CH3)2CHOCH(CH3)2 | 68 | 0.38 | 220 | 1.365 |
Methanol | CH3OH | 65 | 0.54 | 205 | 1.329 |
Methyl ethyl ketone | CH3COC2H5 | 80 | 0.38 | 329 | 1.381 |
n-Propanol | CH3CH2CH2OH | 97 | 1.9 | 240 | 1.385 |
THF | C4H8O | 66 | 0.46 | 212 | 1.408 |
Toluene | C6H5CH3 | 110 | 0.55 | 285 | 1.496 |
Water | H2O | 100 | 0.89 | - | 1.333 |
1,2,4-Trichlorobenzene | C6H3Cl3 | 213 | 1.89 | - | 1.5717 |
o-Dichlorobenzene | C6H4Cl2 | 180 | 1.26 | - | 1.5515 |
Hexafluoroisopropanol | (CF3)2CHOH | 58 | 1.62 | - | 1.275 |
Applications
- Hydrophobic Polymers: THF Eluent
- Hydrophobic Polymers: Chloroform Eluent
- Hydrophobic Polymers: DMF Eluent
- Hydrophobic Polymers: HFIP Eluent
- Hydrophobic Polymers: Other Eluents
- Additives in Hydrophobic Polymers
- Hydrophobic Oligomers
- Hydrophilic Polymers
- Cationic Polymers
- Solvent-peak Separation Columns
Applications (Related Information)
- To Realize Pure SEC Mode
- Guidelines for Shodex Column Selection
- Sample Concentration for SEC Analysis
- Effects of Sample Load (Pullulan)
- Selection of Calibration Curves
- Selection of Calibration Standards
- Connection of Different Pore-size Columns
- Comparison of Calibration Curves: Linear Type and Conventional Type
- Comparison of Separation: Linear Type and Conventional Type (1)
- Comparison of Separation: Linear Type and Conventional Type (2)
- Solubility of Solvents
- Polarities of Solvents