Physical Chemistry Properties of Solvents

Physical chemistry properties of solvents which are commonly used for SEC analysis is shown below.

SolventChemical formulaBoiling
point
(°C)		Viscosity
cp,
(25 °C)		Transmission
limit (nm)		Refractive
index nD25
Acetic acid CH3COOH
118
1.1
-
1.372
Acetonitrile CH3CN
82
0.34
190
1.344
Acetone CH3COCH3
56
0.30
330
1.359
Carbon tetrachloride CCl4
77
0.90
265
1.466
Chloroform CHCl3
61
0.53
245
1.443
Cyclohexane C6H12
81
0.90
200
1.427
Dichloroethane CH2ClCH2Cl
83
0.78
228
1.445
Dichloromethane CH2Cl2
40
0.41
233
1.424
Dimethylformamide (CH3)2NOCH
153
0.80
268
1.428
Dimethylsulfoxide (CH3)2SO
189
2.00
268
1.477
Dioxane C4H8O2
101
1.2
215
1.422
Ethanol C2H5OH
78
1.08
210
1.361
Ethyl acetate CH3COOC2H5
77
0.43
256
1.370
Ethylene glycol HOCH2CH2OH
182
16.5
-
1.431
n-Hexane CH3(CH2)4CH3
69
0.30
190
-
Isopropyl alcohol CH3CH(OH)CH3
82
1.9
205
1.384
Isopropyl ether (CH3)2CHOCH(CH3)2
68
0.38
220
1.365
Methanol CH3OH
65
0.54
205
1.329
Methyl ethyl ketone CH3COC2H5
80
0.38
329
1.381
n-Propanol CH3CH2CH2OH
97
1.9
240
1.385
THF C4H8O
66
0.46
212
1.408
Toluene C6H5CH3
110
0.55
285
1.496
Water H2O
100
0.89
-
1.333
1,2,4-Trichlorobenzene C6H3Cl3
213
1.89
-
1.5717
o-Dichlorobenzene C6H4Cl2
180
1.26
-
1.5515
Hexafluoroisopropanol (CF3)2CHOH
58
1.62
-
1.275

Applications

Applications (Related Information)