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Selection of Calibration Standards

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THF Eluent Generally, polystyrene (PS) standards are used as calibration standards for organic SEC (GPC) with THF eluent.
However, when there is a large difference between the structure of the polymer sample and that of the polystyrene, other standards may be used, if the structure is close to that of the polymer. Poly(ethylene glycol) (PEG) or poly(methyl methacrylate) (PMMA) can be used for this purpose.
Chloroform Eluent PS standards are commonly used for organic SEC (GPC) analysis with chloroform eluent.
DMF Eluent Polyethylene oxide (PEO) and PEG are used for the calibration standards of organic SEC (GPC) analysis with DMF eluent, when using KD-800 columns. If PS is used for the standard, PS elutes slower because PS is adsorbed by the packing material of KD-800 columns.
Different from KD-800 columns, PS as well as PEG and PEO can be used as calibration standards for the SB-800 HQ series with DMF eluent. For samples, which cannot be analyzed by the KD-800 columns because of the adsorption by the packing material, the SB-800 HQ series can be employed.
Toluene Eluent PS is used for the standard for organic SEC (GPC) analysis with toluene eluent.
Quinoline Eluent PEG is used for the standard for organic SEC (GPC) analysis with guinoline eluent.
Methanol Eluent PEO and PEG is used for the standard for organic SEC (GPC) analysis with methanol eluent because they are easily dissolved in methanol.
Aqueous Eluent Pullulan, PEO and PEG are used for Aqueous SEC (GFC) analysis with aqueous eluents, such as pure water or aqueous salt solution.

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