Steroid hormones like estradiol are excreted in animals as aggregates - a form presenting no hormonal activity. It has however been reported that upon removal of water, they disassemble to release the free active form, which has prompted efforts to characterize such aggregates. In this application, estradiol and its metabolic by-products, i.e. 11 compounds including estrone and a variety of estroriol glucuronides were analyzed with the polymer-based Asahipak NH2P-40 2D column in HILIC mode with LC/MS/MS detection.
*Sales of the product used in this application is discontinued, however if you are still interested in this application, please contact us.
Sample : 0.1 ppm each, 5 μL
1. Estrone -3-sulfate
2. β-Estradiol -3-sulfate
3. Estriol -3-sulfate
4. Dehydroepiandrosterone -3-sulfate
5. Estrone -3-glucuronide
6. β-Estradiol -17-glucuronide
7. β-Estradiol -3-glucuronide
8. Estriol -3-glucuronide
9. Estriol -16-glucuronide
10. Dehydroepiandrosterone -3-glucuronide
11. Androsterone -3-glucuronide
*Sales of the product used in this application is discontinued, however if you are still interested in this application, please contact us.
Sample : 0.1 ppm each, 5 μL
1. Estrone -3-sulfate
2. β-Estradiol -3-sulfate
3. Estriol -3-sulfate
4. Dehydroepiandrosterone -3-sulfate
5. Estrone -3-glucuronide
6. β-Estradiol -17-glucuronide
7. β-Estradiol -3-glucuronide
8. Estriol -3-glucuronide
9. Estriol -16-glucuronide
10. Dehydroepiandrosterone -3-glucuronide
11. Androsterone -3-glucuronide
Column : Shodex Asahipak NH2P-40 2D (2.0 mm I.D. x 150 mm) Eluent : A; 10 mM NH4OH CH3CN B; 10 mM NH4OH H2O Gradient : Linear gradient; (B %) 22 % (0 - 5 min), 22 - 70 % (5 - 10 min), 70 % (10 - 15 min) Flow rate : 0.2 mL/min Detector : ESI-MS/MS (Polarity : Negative) Column oven : 40 °CData courtesy of Mr. Atsushi Yamamoto, Osaka City Institute of Public Health and Environmental Sciences
Sample Name Index
Applications
- Adrenal Cortical Hormones (1) (SB-802.5 HQ)
- Adrenal Cortical Hormones (2) (GF-310 HQ)
- Steroides (SB-802.5 HQ)
- Catecholamines (1) (DE-613)
- Catecholamines (3) (ES-502C 7C)
- Catecholamines in Urine (ES-502C 7C)
- Catecholamine and Salsolinol (ES-502C 7C)
- Catecholamines and Metabolites (1) (ODP-50 6D)
- Gibberellin Homologues (ES-502N 7C)
- Gibberellin Isomers (ES-502N 7C)
- Isolation of Ecdyson from Vitex Fiherii (GS-320 20G)
- LC/MS/MS Analysis of Monoamine Neurotransmitters (VC-50 2D)
- LC/MS/MS Analysis of Histamine and Histidine (VC-50 2D)