Five kinds of monoamine neurotransmitters were analyzed using HILICpak VC-50 2D, a polymer based column for HILIC mode. Histamine is more basic than other monoamines and causing a stronger ion exchange interactions between histamine and gel material resulting histamine to elute later.
Sample: 20 µL
0.1 μM each (in H2O)
- Noradrenaline
- Adrenaline
- Dopamine
- Serotonin
- Histamine
- Column
- ：Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm)
- Eluent
- ：(A); 200 mM HCOOH aq. (B); CH3CN
Linear gradient (High pressure); B % = 60 % (0 to 5 min), 60 % to 10 % (5 to 6 min), 10 % (6 to 20 min) - Flow rate
- ：0.3 mL/min
- Detector
- ：ESI-MS/MS (MRM Positive)
- Column temp.
- ：40 ℃
Sample Name Index
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Product Name Index
Applications
- Adrenal Cortical Hormones (1) (SB-802.5 HQ)
- Adrenal Cortical Hormones (2) (GF-310 HQ)
- Steroides (SB-802.5 HQ)
- Catecholamines (1) (DE-613)
- Catecholamines (3) (ES-502C 7C)
- Catecholamines in Urine (ES-502C 7C)
- Catecholamine and Salsolinol (ES-502C 7C)
- Catecholamines and Metabolites (1) (ODP-50 6D)
- Gibberellin Homologues (ES-502N 7C)
- Gibberellin Isomers (ES-502N 7C)
- Isolation of Ecdyson from Vitex Fiherii (GS-320 20G)
- LC/MS/MS Monitoring of the Estradiol Metabolism (NH2P-40 2D)
- LC/MS/MS Analysis of Histamine and Histidine (VC-50 2D)