Five kinds of monoamine neurotransmitters were analyzed using HILICpak VC-50 2D, a polymer based column for HILIC mode. Histamine is more basic than other monoamines and causing a stronger ion exchange interactions between histamine and gel material resulting histamine to elute later.


Sample :
0.1 μM each (in H₂O), 20 μL
Noradrenaline
Adrenaline
Dopamine
Serotonin
Histamine

Column       : Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm)
Eluent       : (A) 200 mM HCOOH aq. 
               (B) CH3CN    
               Linear gradient (High pressure) ;
               B % = 60 % (0 to 5 min) 
                    60 % to 10 % (5 to 6 min)
                    10 % (6 to 20 min) 
Flow rate    : 0.3 mL/min
Detector     : ESI-MS/MS (MRM Positive)
Column temp. : 40 ℃