Catecholamines and metabolites were analyzed using Asahipak ODP-50 6D.


Sample : Catecholamines
NE: Norepinephrine
E: Epinephrine
DOPA: 3,4-Dihydroxyphenylalanine
DA: Dopamine
5HT: 5-Hydroxytryptamine
DOMA: 3,4-Dihydroxymandelic acid
MHPG: 3-Methoxy-4-hydroxyphenylglycol
VMA: Vanillylmandelic acid
DOPAC: 3,4-Dihydroxyphenylacetic acid
HVA: Homovanilic acid
5HIAA: 5-Hydroxyindole-3-acetic acid

Column       : Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent       : (A); 0.1 M H3PO4 (pH2.0)
               (B); 0.1 M H3PO4 (pH2.0)/CH3CN=70/30
               Linear gradient:
               0 min to 10 min, (A)
               afetr 10 min, 2.5 %/min gradient of (B)
Flow rate    : 0.8 mL/min
Detector     : UV (280 nm)