Catecholamines and Metabolites (1) (ODP-50 6D)

Catecholamines and metabolites were analyzed using Asahipak ODP-50 6D.

Sample: Catecholamines

  1. NE: Norepinephrine                           MHPG: 3-Methoxy-4-hydroxyphenylglycol
  2. E: Epinephrine                                   VMA: Vanillylmandelic acid
  3. DOPA: 3,4-Dihydroxyphenylalanine  DOPAC: 3,4-Dihydroxyphenylacetic acid
  4. DA: Dopamine                                   HVA: Homovanilic acid
  5. 5HT: 5-Hydroxytryptamine                 5HIAA: 5-Hydroxyindole-3-acetic acid
  6. DOMA: 3,4-Dihydroxymandelic acid
  7.  

 

Column
Shodex Asahipak ODP-50 6D (6.0 mm I.D. x 150 mm)
Eluent
(A); 0.1 M H3PO4 (pH2.0)
(B); 0.1 M H3PO4 (pH2.0)/CH3CN=70/30
Linear gradient: 0 min to 10 min, (A) after 10 min, 2.5 %/min gradient of (B)
Flow rate
0.8 mL/min
Detector
UV(280 nm)

 

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