Hemoglobin A, F, S and C were analyzed using IEC SP-FT 4A, a non-porous type column for cation exchange chromatography. SP-FT 4A can provide ultra-rapid and high-resolution analysis using conventional devices as same as when using UHPLC.
Sample: 5 μL
AFSC Hemoglobin Control (51-fold diluted)
- Column
- ：Shodex IEC SP-FT 4A (4.6 mm I.D. x 10 mm)
- Eluent
- ：(A); 20 mM *MES buffer (pH5.6)
(B); (A) + 0.5 M Na2SO4
*MES : 2-(N-Morpholino)ethanesulfonic acid
Linear gradient; 5 % (B) to 100 % (B) (2 min) - Flow rate
- ：1.7 mL/min
- Detector
- ：VIS(415 nm)
- Column temp.
- ：30 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
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