Ultra-rapid Analysis of Standard Proteins (SP-FT 4A)

Five kinds of standard proteins were analyzed using IEC SP-FT 4A, a non-porous type column for cation exchange chromatography. These proteins can be separated well at 3.0 mL/min flow rate while maintaining the same separation at 1.5 mL/min flow rate. This analysis can be completed, within a mere 50 seconds.
(Caution) Backpressure will differ depends on the LC system used and the analytical conditions such as eluent compositions and column temperature. Please set the system pressure (sum of column and system pressures) less than 20 MPa.


Sample : 5 μL
1. Ovalbumin 0.8 mg/mL
2. Trypsinogen 1.6 mg/mL
3. Ribonuclease A 1.6 mg/mL
4. Cytochrome c 0.8 mg/mL
5. Lysozyme 0.4 mg/mL


Column       : Shodex IEC SP-FT 4A (4.6 mm I.D. x 10 mm)
Eluent       : (A); 20 mM *MES buffer (pH6.0)
               (B); (A) + 0.5 M Na2SO4
               Linear gradient: (A) to (B) (2 min)     
Flow rate    : 1.5, 3.0 mL/min
Detector     : UV (280 nm)
Column temp. : Room temp.
*MES : 2-(N-Morpholino) ethanesulfonic acid

Sample Name Index

Operation Manual / Certificate of Analysis

Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.

Product Name Index

Applications