The effects of flow rate for protein analysis with IEC SP-FT 4A, a column for cation exchange chromatography, were investigated. The particle size of SP-FT 4A is small (2.7 μm) but the pressure of the column can be suppressed by advanced control for particle size. Since the pressure is under 20 MPa at 3.0 mL/min flow rate, SP-FT 4A can provide ultra-rapid and high-resolution analysis using conventional devices as same as when using UHPLC.
Sample: 5 μL
1 mg/mL each
-
- 1.α-Chymotrypsinogen A
- 2.Ribonuclease A
- Column
- ：Shodex IEC SP-FT 4A (4.6 mm I.D. x 10 mm ; 2.7 μm)
- Eluent
- ：(A); 20 mM *MES buffer (pH5.6)
(B); (A) + 0.5 M Na2SO4 *MES : 2-(N-Morpholino)ethanesulfonic acid
Linear gradient; (A) to (B) (2 min) - Flow rate
- ：1.0 mL/min, 1.5 mL/min, 3.0 mL/min
- Detector
- ：UV (280 nm)
- Column temp.
- ：Room temp.
Sample Name Index
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