When testing the repeatability of Asahipak ODP-50 4E, a polymer-based column for reversed phase chromatography, under alkaline condition, azithromycin was injected repeatedly according toUSP 39 method. When theoretical plate number for azithromycin and resolution of azithromycin/azaerythromycin A regarding number of injections were measured, theoretical plate number and resolution were not changed after 3,000 injections. It was confirmed that ODP-50 4E is very alkaline-resistant.
Sample: 10 μL
0.5 mg/mL each
- 1.Azaerythromycin A
- 2.Azithromycin
dissolved in 6.7 g/L Dibasic potassium phosphate aq. (pH8.0 adjusted with phosphoric acid)/CH3CN=40/60
- Column
- ：Shodex Asahipak ODP-50 4E (4.6mmI.D. x 250mm)
- Eluent
- ：6.7 g/L Dibasic potassium phosphate aq. (pH11.0 adjusted with 10 M KOH)/CH3CN=40/60
- Flow rate
- ：1.0 mL/min
- Detector
- ：UV (210 nm)
- Column temp.
- ：40 ℃
Operation Manual / Certificate of Analysis
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