The relation between column temperature and retention time and the relation between column temperature and theoretical plate number (TPN) for ODP2 HP 4D (4.6 mm I.D. x 150 mm length) are shown. As retention time and TPN vary with changes in column temperature, the use of a column oven for temperature control is recommended.


Sample : 5 μL
Phenol 300 μg/mL
Methyl bezoate 350 μg/mL
Toluene 1000 μg/mL
Ratio of TPN [%] : TPN at 40 °C is set as 100%.

Column       : Shodex ODP2 HP-4D (4.6 mm I.D. x 150 mm)
Eluent       : H2O/CH3CN=55/45
Detector     : UV (254 nm)