Effects of Column Temperature (ODP2 HP)

The relation between column temperature and retention time and the relation between column temperature and theoretical plate number (TPN) for ODP2 HP 4D (4.6 mm I.D. x 150 mm length) are shown. As retention time and TPN vary with changes in column temperature, the use of a column oven for temperature control is recommended.

Sample: 5 μL

  1. Phenol 300 ppm
  2. Methyl benzoate 350 ppm
  3. Toluene 1000 ppm

* Ratio of TPN [%] : TPN at 40 ℃ is set as 100%.

 

  1.  
Column
Shodex ODP2 HP-4D (4.6 mm I.D. x 150 mm)
Eluent
H2O/CH3CN=55/45
Flow rate
0.5 mL/min
Detector
UV (254 nm)

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