The relation between sample loads and thepretical plate number (TPN) for ODP2 HP 4D (4.6 mm I.D. x 150 mm length) is shown. Sample loads of 10 μg or less are recommended with ODP2 HP-4D for best column performance and sample loads of 2 μg or below are recommended for ODP2 HP-2D (2 mm I.D. x 150 mm length).


Sample : 5 μL
Phenol
Methyl bezoate
Caffeine
Ratio of TPN [%] : TPN at sample load of 5 μg is set as 100%.

Column       : Shodex ODP2 HP-4D (4.6 mm I.D. x 150 mm)
Eluent       : H2O/CH3CN=55/45
Flow rate    : 1.0 mL/min
Detector     : UV (254 nm) Phenol, Methyl benzoate
               UV (300 nm) Caffeine
Column temp. : 40 °C