The relation between sample injection volume and theoretical plate number (TPN) for ODP2 HP 4D (4.6 mm I.D. x 150 mm length) is shown. Sample injection volume of 40 μL and below is recommended for ODP2 HP-4D to achieve maximum efficiency. Sample injection volume of 8 μL and below is recommended for ODP2 HP-2D (2 mm I.D. x 150 mm length).
Sample: 0.6 µg each
- Phenol
- Methyl benzoate
- Caffeine
* Ratio of TPN [%] : TPN at sample injection of 2 μL is set as 100%.
- Column
- ：Shodex ODP2 HP-4D (4.6 mm I.D. x 150 mm)
- Eluent
- ：H2O/CH3CN=55/45
- Flow rate
- ：1.0 mL/min
- Detector
- ：UV (254 nm) Phenol, Methyl benzoate
UV (300 nm) Caffeine - Column temp.
- ：40 ℃
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.