The relation between sample injection volume and theoretical plate number (TPN) for ODP2 HP 4D (4.6 mm I.D. x 150 mm length) is shown. Sample injection volume of 40 μL and below is recommended for ODP2 HP-4D to achieve maximum efficiency. Sample injection volume of 8 μL and below is recommended for ODP2 HP-2D (2 mm I.D. x 150 mm length).


Sample : 0.6 μg each
Phenol
Methyl bezoate
Caffeine
Ratio of TPN [%] : TPN at sample injection of 2 μL is set as 100%.

Column       : Shodex ODP2 HP-4D (4.6 mm I.D. x 150 mm)
Eluent       : H2O/CH3CN=55/45
Flow rate    : 1.0 mL/min
Detector     : UV (254 nm) Phenol, Methyl benzoate
               UV (300 nm) Caffeine
Column temp. : 40 °C