Five kinds of monoamine neurotransmitters were analyzed using HILICpak VC-50 2D, a polymer based column for HILIC mode. Histamine is more basic than other monoamines and causing a stronger ion exchange interactions between histamine and gel material resulting histamine to elute later.
Sample :
0.1 μM each (in H2O), 20 μL
Noradrenaline
Adrenaline
Dopamine
Serotonin
Histamine
Column : Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm) Eluent : (A) 200 mM HCOOH aq. (B) CH3CN Linear gradient (High pressure) ; B % = 60 % (0 to 5 min) 60 % to 10 % (5 to 6 min) 10 % (6 to 20 min) Flow rate : 0.3 mL/min Detector : ESI-MS/MS (MRM Positive) Column temp. : 40 ℃
Sample Name Index
Operation Manual / Certificate of Analysis
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Applications
- Adrenal Cortical Hormones (1) (SB-802.5 HQ)
- Adrenal Cortical Hormones (2) (GF-310 HQ)
- Steroides (SB-802.5 HQ)
- Catecholamines (1) (DE-613)
- Catecholamines (3) (ES-502C 7C)
- Catecholamines in Urine (ES-502C 7C)
- Catecholamine and Salsolinol (ES-502C 7C)
- Catecholamines and Metabolites (1) (ODP-50 6D)
- Gibberellin Homologues (ES-502N 7C)
- Gibberellin Isomers (ES-502N 7C)
- Isolation of Ecdyson from Vitex Fiherii (GS-320 20G)
- LC/MS/MS Monitoring of the Estradiol Metabolism (NH2P-40 2D)
- LC/MS/MS Analysis of Histamine and Histidine (VC-50 2D)