Basically, Asahipak GF-310 HQ is a Aqueous/Organic (multi-solvent use) SEC column. Here, the column was used for the analysis of adrenal cortical hormones by multimode with a mixture of water/acetonitrile as an eluent.


Sample : 50 μL
1. Aldosterone 0.125 mg/mL
2. Corticosterone 0.25 mg/mL
3. Deoxycorticosterone 0.25 mg/mL

Column       : Shodex Asahipak GF-310 HQ (7.5 mm I.D. x 300 mm)
Eluent       : H2O/CH3CN=55/45
Flow rate    : 0.5 mL/min
Detector     : UV (240 nm)
Column temp. : 30 °C