Basically, OHpak SB-802.5 HQ is a GFC column. Here, the column was used for the analysis of adrenal cortical hormones by reversed phase mode with a mixture of water/acetonitrile as an eluent.


Sample : Steroides mixture
1. Aldosterone
2. Corticosterone
3. Deoxycorticosterone

Column       : Shodex OHpak SB-802.5 HQ (8.0 mm I.D. x 300 mm)
Eluent       : H2O/CH3CN=70/30
Flow rate    : 1.0 mL/min
Detector     : UV (240 nm)
Column temp. : 30 °C