Histamine is a neurotransmitter and it also is well known as a causative agent of food poisoning. In this application, HILICpak VC-50 2D was used for a LC/MS/MS analysis of histidine which is precursor of histamine. The two components can be simultaneously analyzed under acidic conditions.
Sample : 20 μL
5 ng/mL each (in H2O)
Histidine
Histamine
Column : Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm) Eluent : 250 mM HCOOH aq./CH3CN=70/30 Flow rate : 0.3 mL/min Detector : ESI-MS/MS (MRM Positive) Column temp. : 40 °C
Sample Name Index
Operation Manual / Certificate of Analysis
Operation Manuals and Certificate of Analysis / Inspection Certificate for the following products can be downloaded here.
Product Name Index
Applications
- Adrenal Cortical Hormones (1) (SB-802.5 HQ)
- Adrenal Cortical Hormones (2) (GF-310 HQ)
- Steroides (SB-802.5 HQ)
- Catecholamines (1) (DE-613)
- Catecholamines (3) (ES-502C 7C)
- Catecholamines in Urine (ES-502C 7C)
- Catecholamine and Salsolinol (ES-502C 7C)
- Catecholamines and Metabolites (1) (ODP-50 6D)
- Gibberellin Homologues (ES-502N 7C)
- Gibberellin Isomers (ES-502N 7C)
- Isolation of Ecdyson from Vitex Fiherii (GS-320 20G)
- LC/MS/MS Monitoring of the Estradiol Metabolism (NH2P-40 2D)
- LC/MS/MS Analysis of Monoamine Neurotransmitters (VC-50 2D)