Asahipak ODP-50 6D （反相色谱）分析肌红蛋白的胰蛋白酶。
Sample : Tryptic digest of Myoglobin
(肌红蛋白的氨基酸序列)
| 氨基酸缩写 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| A | Ala | C | Cys | D | Asp | E | Glu | F | Phe |
| G | Gly | H | His | I | Ile | K | Lys | L | Leu |
| M | Met | N | Asn | P | Pro | Q | Gln | R | Arg |
| S | Ser | T | Thr | V | Val | W | Trp | Y | Tyr |
Column : Shodex Asahipak ODP-50 6D (6. 0mm I.D. x 150 mm)
Eluent : (A); 0.07 % TFA aq.
(B); CH3CN containing 0.05 % TFA
Initial ratio (A)/(B)=90/10
Linear gradient of 1 %/min
Flow rate : 0.8 mL/min
Detector : UV (214 nm)
Column temp. : 30 ℃
Coutesy of Dr. Takano, Institute for Protein Research, Osaka University