10种标准蛋白质通过 PROTEIN KW-804 (水溶性色谱柱 SEC(GFC))进行分析。


Sample :
1. Thyroglobulin (MW: 669,000)
2. Aldolase (MW: 158,000)
3. BSA (MW: 67,000)
4. Ovalbumin (MW: 43,000)
5. Peroxidase (MW: 40,200)
6. Adenylate kinase (MW: 32,000)
7. Myoglobin (MW: 17,000)
8. Ribonuclease A (MW: 13,700)
9. Aprotinin (MW: 6,500)
10. Vitamin B₁₂ (MW: 1,350)

Column       : Shodex PROTEIN KW-804 (8.0 mm I.D. x 300 mm)
Eluent       : 50 mM Sodium phosphate buffer (pH7.0) + 0.3 M NaCl
Flow rate    : 1.0 mL/min
Detector     : UV (220 nm)
Column temp. : Room temp.