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LC/MS Analysis of Iodide (VT-50 2D)


​​Iodide is an essential element in the human body to make thyroid hormone. Iodide was analyzed using HILICpak VT-50 2D, a polymer-based HILIC mode column, with LC/MS detection. High acetonitrile concentration in the eluent is suitable for the analysis with higher sensitivity and selectivity than ion chromatography.

Sample : 5μL
1. I-, Iodide 1ng/mL (in H2O)

Column       : Shodex HILICpak VT-50 2D (2.0mmI.D. x 150mm)
Eluent       : 50mM HCOONH4 aq./CH3CN=20/80
Flow rate    : 0.3mL/min
Detector     : ESI-MS (SIM Negative)
Column temp. : 40°C

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