HILICpak VC-50 2D was used to separate trivalent chrome (Cr³⁺) and hexavalent chrome (CrO₄^2-). The gel made from polyvinyl alcohol base material modified with carboxyl functional group is packed in VC-50. In this analysis, trivalent chrome is retained mainly by ion-exchange effects while hexavalent chrome is mainly retained by hydrophobic interaction and they were well separated. The linearity of calibration curves for both ions were confirmed.


Sample : 10 μg/L each, 25 μL
1. Cr(VI) from K₂CrO₄
2. Cr(III) from Cr(NO₃)₃
^*diluted with eluent

Column       : Shodex HILICpak VC-50 2D (2.0 mm I.D. x 150 mm)
Eluent       : 9 mM HNO3 aq.
Flow rate    : 0.2 mL/min
Detector     : ICP-MS SIM m/z 52 　*Helium was used as reagent gas
Column temp. : 40 °C