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Chiral Separation of Norepinephrine (CDBS-453)

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Analysis of norepinephrine (racemic) was performed using ORpak CDBS-453, a chiral separation column.
The analysis conditions for determining the optical purity of epinephrine injection described at in-Process Revision of Pharmacopoeia Forum 44 (6) of the United States Pharmacopeia (USP) were applied.


Sample : 10 μL
Norepinephrine (racemic mixture) 20 μg/mL (in Solution A)


Column       : Shodex ORpak CDBS-453 (4.6 mm I.D. x 150 mm)
Eluent       : *1Solution A/CH3CN=99/1
Flow rate    : 0.3 mL/min
Detector     : UV (280 nm)
Column temp. : 25 °C
*1 : 0.75 g/L Ammonium acetate aqueous solution adjusted to pH 4.0 
     with Glacial acetic acid

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