Epinephrine was analyzed using ORpak CDBS-453, a chiral separation column. Under these analytical conditions, resolution between (R)-Epinephrine and (S)-Epinephrine achieved was more than 1.5.
Sample: 10 μL
Epinephrine hydrochloride 20 μg/mL (in *Solution A)
- 1.(R)-Epinephrine
- 2.(S)-Epinephrine
- Column
- ：Shodex ORpak CDBS-453 (4.6 mm I.D. x 150 mm)
- Eluent
- ：*Solution A/CH3CN=99/1
*0.75 g/L Ammonium acetate aqueous solution adjusted to pH 4.0 with Glacial acetic acid - Flow rate
- ：0.3 mL/min
- Detector
- ：UV (280 nm)
- Column temp.
- ：25 ℃
Sample Name Index
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