Epinephrine was analyzed using ORpak CDBS-453, a column for chiral separation.


Sample : 20 μL
Epinephrine (racemic mixture)
50 μg/mL (in eluent)

Column       : Shodex ORpak CDBS-453 (4.6 mm I.D. x 150 mm)
Eluent       : (0.05 % (w/v) CH3COOH + 0.2 M NaCl) aq./CH3CN=95/5
Flow rate    : 0.5 mL/min
Detector     : UV (280 nm)
Column temp. : 10 °C